Simultaneous epitope and transcriptome measurement in single cells.

TitleSimultaneous epitope and transcriptome measurement in single cells.
Publication TypeJournal Article
Year of Publication2017
AuthorsStoeckius, M, Hafemeister, C, Stephenson, W, Houck-Loomis, B, Chattopadhyay, PK, Swerdlow, H, Satija, R, Smibert, P
JournalNat Methods
Volume14
Issue9
Pagination865-868
Date Published2017 Sep
ISSN1548-7105
KeywordsEpitope Mapping, Epitopes, Gene Expression Profiling, High-Throughput Nucleotide Sequencing, Sequence Analysis, RNA, Tissue Array Analysis, Transcriptome
Abstract

High-throughput single-cell RNA sequencing has transformed our understanding of complex cell populations, but it does not provide phenotypic information such as cell-surface protein levels. Here, we describe cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), a method in which oligonucleotide-labeled antibodies are used to integrate cellular protein and transcriptome measurements into an efficient, single-cell readout. CITE-seq is compatible with existing single-cell sequencing approaches and scales readily with throughput increases.

DOI10.1038/nmeth.4380
PubMed ID28759029
PubMed Central IDPMC5669064
Grant ListDP2 HG009623 / HG / NHGRI NIH HHS / United States
P30 CA016087 / CA / NCI NIH HHS / United States
UM1 HG008901 / HG / NHGRI NIH HHS / United States