@article {26, title = {Simultaneous epitope and transcriptome measurement in single cells.}, journal = {Nat Methods}, volume = {14}, year = {2017}, month = {2017 Sep}, pages = {865-868}, abstract = {

High-throughput single-cell RNA sequencing has transformed our understanding of complex cell populations, but it does not provide phenotypic information such as cell-surface protein levels. Here, we describe cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), a method in which oligonucleotide-labeled antibodies are used to integrate cellular protein and transcriptome measurements into an efficient, single-cell readout. CITE-seq is compatible with existing single-cell sequencing approaches and scales readily with throughput increases.

}, keywords = {Epitope Mapping, Epitopes, Gene Expression Profiling, High-Throughput Nucleotide Sequencing, Sequence Analysis, RNA, Tissue Array Analysis, Transcriptome}, issn = {1548-7105}, doi = {10.1038/nmeth.4380}, author = {Stoeckius, Marlon and Hafemeister, Christoph and Stephenson, William and Houck-Loomis, Brian and Chattopadhyay, Pratip K and Swerdlow, Harold and Satija, Rahul and Smibert, Peter} }